A ferromagnetic tetranuclear CuII complex: catecholase and phenoxazinone synthase activities
نویسندگان
چکیده
منابع مشابه
Phenoxazinone synthase activity of a mononuclear cobalt(III) complex
Metalloenzymes that activate molecular oxygen possess great potential as catalyst for specific oxidation reactions and as guides for the development of efficient small molecule catalysts. These lowmolecular metal complexes serves as functional model and catalyze the same (or a closely related) reaction as does the metalloenzyme to be mimicked. In combination with the structural models which mim...
متن کاملPreparation and characterization of a highly stable phenoxazinone synthase nanogel
BACKGROUND Phenoxazinone synthase (PHS) is a laccase-like multicopper oxidase originating from Streptomyces with great industrial application potential. In this paper, we prepared the PHS nanogel retaining 82 % of its initial activity by aqueous in situ polymerization at pH 9.3. RESULTS The average diameter of the PHS nanogel was 50.8 nm based on dynamic light scattering (DLS) analysis. Fluor...
متن کاملAnisotropic exchange in a tetranuclear Co complex
Very high-frequency (50–715 GHz) electron paramagnetic resonance (EPR) studies of the tetranuclear Co complex [Co(hmp)(dmb)Cl]4 (1), where dmb is 3,3-dimethyl-1-butanol and hmp is the monoanion of 2-hydroxy-methylpyridine, reveal the presence of significant zero-field-splitting (ZFS) within the ground state spin multiplet. Meanwhile, low-temperature hysteresis measurements of 1 (and related Co ...
متن کاملMolecular cloning and in vitro expression of a silent phenoxazinone synthase gene from Streptomyces lividans.
Phenoxazinone synthase (PHS) catalyzes a step in actinomycin D biosynthesis in Streptomyces antibioticus. Two sequences from Streptomyces lividans that hybridize to the phs gene of S. antibioticus have been cloned in Escherichia coli K-12 using the plasmid pBR322. Although there was some similarity in the restriction maps of the two cloned fragments, neither insert appeared to be a direct subse...
متن کاملActinomycin production persists in a strain of Streptomyces antibioticus lacking phenoxazinone synthase.
Truncated fragments of the phenoxazinone synthase gene, phsA, were prepared by the PCR. The resulting fragments were cloned into conjugative plasmid pKC1132 and transferred to Streptomyces antibioticus by conjugation from Escherichia coli. Two of the resulting constructs were integrated into the S. antibioticus chromosome by homologous recombination, and each of the resulting strains, designate...
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ژورنال
عنوان ژورنال: Acta Crystallographica Section A Foundations and Advances
سال: 2017
ISSN: 2053-2733
DOI: 10.1107/s205327331708648x